Efficacy in vitro Antioxidation and in vivo Skin Barrier Recovery of Composition Containing Mineral-cation-phyto DNA Extracted from Aloe vera Adventitious Root

Purpose: This study aimed to extract phyto DNA from the adventitious root of Aloe vera and confirm its role in improving regenerating skin antiaging properties caused by blue light.Methods: Phyto was extracted a complex containing polylysine Poly (amidoamine) made. The antioxidant activity confirmed using DPPH method. MTT test performed on fibroblasts. expression SOD1, FLG, LOR, COL1A1, MMP1 measured RT–PCR after light irradiation HaCaT cells. wound-healing performed. A cream formulation created. Using this method, epidermal water loss determined human application.Results: At 0.85% cation polymer–phyto concentration, 100% fibroblasts survived. 0.1% effect 93%. After being exposed light, only 40.4% survived, but treated with 100 μg/mL complex, 80.5% mineral–cation-phyto ratio 10:1, barrier factors highest. About 95.7% cells regenerated at 10:1 mineral comparison minerals without DNA.Conclusion: aloe vera’s has no cytotoxicity, excellent free radical scavenging ability, can mitigate cytotoxicity. It improve function increasing via synergistic action specific composition DNA, cationic polymer, complexes.